Cat. No: TR 118
Volume : 200ml
Storage: Store at 4 ℃- 25 ℃
TRI REAGENT® is a complete and ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and
proteins from samples of human, animal, plant, yeast, bacterial and viral origin. TRI REAGENT is the improved version of the popular
single-step method of total RNA isolation (1, 2). This highly reliable technique performs well with small and large quantities of tissues
or cultured cells, and allows simultaneous processing of a large number of samples. TRI REAGENT and the single-step method are
subjects of the international patents.
TRI REAGENT combines phenol and guanidine thiocyanate in a ono-phase solution to facilitate the immediate and most effective
inhibition of RNase activity. A biological sample is homogenized or lysed in TRI REAGENT and the homogenate is separated into
aqueous and organic phases by bromochloropropane or chloroform addition and centrifugation. RNA remains exclusively in the
aqueous phase, DNA in the interphase, and proteins in the organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol,
washed with ethanol and solubilized. DNA and proteins are sequentially precipitated from the interphase and organic phase with ethanol and
isopropanol, washed with ethanol and solubilized.
STABILITY: TRI REAGENT is stable at 25 C for at least two years from the date of purchase (3).
The protocol includes the following steps
1. HOMOGENIZATION - 1 ml TRI REAGENT + 50 - 100 mg tissue, 5 - 10 x 106 cells or 10 cm2 of culture plate.
2. PHASE SEPARATION - homogenate + 0.1 ml BCP or 0.2 ml chloroform.
3. RNA PRECIPITATION - aqueous phase + 0.5 ml isopropanol.
4. RNA WASH - 1 ml 75% ethanol.
5. RNA SOLUBILIZATION - FORMAzol®, 0.5% SDS, or water.
The procedure is carried out at room temperature, unless stated otherwise.
상호 : (주)제노믹베이스
대표자 : 윤찬도,손대연,이정민
사업자등록번호 : 204-86-02735
개인정보관리자 : 윤찬도
팩스 : 02-2215-4929
이메일 : firstname.lastname@example.org
주소 : 서울특별시 동대문구 장한로17길 9 (장안동 공감대빌딩) 4층
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